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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
22/02/2021 |
Actualizado : |
22/02/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
LEONI, C.; DUARTE, F.; SPERONI, G.; SILVERA, M.; IRIARTE, W.; BONNECARRERE, V. |
Afiliación : |
CAROLINA LEONI VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FELICIA DUARTE BAREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; G. SPERONI, Departamento de Biolog??a Vegetal, Facultad de Agronom??a, Universidad de la Repu?blica, Montevideo, Uruguay.; MARIANA SILVERA ORREGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WANDA IRIARTE GRECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Etiology of pear flower bud necrosis in Uruguay. [Conference paper]. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Acta Horticulturae, February 2021, N°1303, p. 359-366. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.50 |
ISSN : |
0567-7572 (print); 2406-6168 (electronic) |
DOI : |
10.17660/ActaHortic.2021.1303.50 |
Idioma : |
Inglés |
Notas : |
Article history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. |
Contenido : |
Abstract:
Pear flower bud necrosis (PFBN) has been observed in Uruguayan orchards affecting the productivity of the trees. Severity of the problem is highly variable across years and despite the fact that it is observed in several countries, the etiology of the disease is not always clear. To elucidate the cause of PFBN, different activities were implemented over several years: surveys of pear orchards to quantify PFBN severity, morphological and histological studies of diseased buds, and isolation of potential pathogenic microorganisms from affected tissues. From the orchard surveys no clear association between climatic conditions (mean temperatures, precipitation, chilling hours) and PFBN were detected for the five years evaluated. Flower buds collected one month before flowering and fixed in formalin-ethanol-acetic acid solution showed necrosis associated with tumour development in bud primordia, and histological preparations from those tumours showed cell hypertrophy and hyperplasia. Finally, from the diseased buds we consistently isolated bacteria and no fungi. The bacteria were gram negative, oxidase positive, fluorescent on King B medium, produced indole-3-acetic acid and were positive in the tobacco hypersensitivity test. We sequenced the 16S RNA genes of the isolated bacteria to perform a taxonomic classification. We conclude that PFBN in Uruguay is caused by bacteria belonging to the Pseudomonas genus.
@ International Society for Horticultural Science. |
Palabras claves : |
WILLIAM'S. |
Thesagro : |
PSEUDOMONAS SP; PYRUS COMMUNIS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02505naa a2200253 a 4500 001 1061746 005 2021-02-22 008 2021 bl uuuu u00u1 u #d 022 $a0567-7572 (print); 2406-6168 (electronic) 024 7 $a10.17660/ActaHortic.2021.1303.50$2DOI 100 1 $aLEONI, C. 245 $aEtiology of pear flower bud necrosis in Uruguay. [Conference paper].$h[electronic resource] 260 $c2021 500 $aArticle history: Published 5 February 2021. In: Acta Horticulturae (ISHS) 1303: XIII International Pear Symposium, Montevideo, Uruguay. Conveners: Roberto Zoppolo, Danilo Cabrera. Editors: Roberto Zoppolo, Danilo Cabrera, D. Granatstein. 520 $aAbstract: Pear flower bud necrosis (PFBN) has been observed in Uruguayan orchards affecting the productivity of the trees. Severity of the problem is highly variable across years and despite the fact that it is observed in several countries, the etiology of the disease is not always clear. To elucidate the cause of PFBN, different activities were implemented over several years: surveys of pear orchards to quantify PFBN severity, morphological and histological studies of diseased buds, and isolation of potential pathogenic microorganisms from affected tissues. From the orchard surveys no clear association between climatic conditions (mean temperatures, precipitation, chilling hours) and PFBN were detected for the five years evaluated. Flower buds collected one month before flowering and fixed in formalin-ethanol-acetic acid solution showed necrosis associated with tumour development in bud primordia, and histological preparations from those tumours showed cell hypertrophy and hyperplasia. Finally, from the diseased buds we consistently isolated bacteria and no fungi. The bacteria were gram negative, oxidase positive, fluorescent on King B medium, produced indole-3-acetic acid and were positive in the tobacco hypersensitivity test. We sequenced the 16S RNA genes of the isolated bacteria to perform a taxonomic classification. We conclude that PFBN in Uruguay is caused by bacteria belonging to the Pseudomonas genus. @ International Society for Horticultural Science. 650 $aPSEUDOMONAS SP 650 $aPYRUS COMMUNIS 653 $aWILLIAM'S 700 1 $aDUARTE, F. 700 1 $aSPERONI, G. 700 1 $aSILVERA, M. 700 1 $aIRIARTE, W. 700 1 $aBONNECARRERE, V. 773 $tActa Horticulturae, February 2021, N°1303, p. 359-366. DOI: https://doi.org/10.17660/ActaHortic.2021.1303.50
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